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Second harmonic generation microscopy of the living human eye

Visualizing In Vivo Human Ocular Tissues with Two-Photon Microscopy


Visualization and analysis of transparent living ocular tissues at microscopic scale have been a challenge during the last decades. Nowadays clinical devices only allow imaging cellular corneal structures; however, collagen fibers of the stroma (90 % of the corneal thickness) cannot be imaged. It has been recently reported a compact Second Harmonic Generation (SHG) microscope able to image the living human cornea and the sclera for the very first time.

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