Making Multiphoton Microscopy More Useful
Lynn Savage, Features Editor, email@example.com
Multiphoton microscopy techniques – including two-photon fluorescence and single-harmonic generation (SHG) – have blossomed over the more than 20 years since they first became widespread, but they continue to evolve, becoming more useful than ever to biologists. Much of the new work being performed with multiphoton techniques is additive: using adaptive optics, for example, or combining it with an adjunct methodology such as coherent anti-Stokes Raman scattering (CARS)-based spectroscopy. The following are a few examples of exciting work centered on these multimodal methods.