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Polarization and Nonlinear Microscopy. Image Improvement of Ocular Tissue


2-photon imaging of collagen-based biological tissues might benefit from the choice of particular incident polarization states. Here we further explore the effects of polarization on the visualization of ocular structures. Theoretical and experimental results suggest that linear polarization optimizes the imaging of samples containing mostly parallel elongated structures. However this type of polarization might not be the most appropriate when tissues present random distributed collagen patterns.

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