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Adaptive Optics Multiphoton Microscopy of ex-vivo Ocular Tissues

PURPOSE

To develop an improved version of a multiphoton microscope incorporating the control of the beam wavefront, wavelength and polarization. The instrument will be primarily applied to obtain two-photon autofluorescence (TPEF) and second harmonic generation (SHG) images of ex-vivo retinal and corneal tissues of fish, bovine and porcine eyes.

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